RESUMO
Microgravity is one of the most common causes counting for the bone loss. Mesenchymal stem cells (MSCs) contribute greatly to the differentiation and function of bone related cells. The development of novel MSCs biomarkers is critical for implementing effective therapies for microgravity induced bone loss. We aimed to find the new molecules involved in the differentiation and function of MSCs in mouse simulated microgravity model. We found CD226 was preferentially expressed on a subset of MSCs. Simulation of microgravity treatment significantly increased the proportion of CD226+ Lin- CD117- Sca1+ MSCs. The CD226+ MSCs produced higher IL-6, M-CSF, RANKL and lower CD200 expression, and promoted osteoclast differentiation. This study provides pivotal information to understand the role of CD226 in MSCs, and inspires new ideas for prevention of bone loss related diseases.
Assuntos
Células-Tronco Mesenquimais , Ausência de Peso , Animais , Camundongos , Ausência de Peso/efeitos adversos , Células-Tronco Mesenquimais/metabolismo , Diferenciação Celular/fisiologia , Células Cultivadas , Simulação de Ausência de PesoRESUMO
The black-winged fly, Felderimyia fuscipennis (Diptera: Tephritidae), is an insect pest of bamboo shoot, mainly distributed in Thailand, Malaysia and Yunnan Province and Guangxi Autonomous Region, China. The complete sequence of the mitogenome of F. fuscipennis has been determined in this study. The whole mitogenome sequence is 16,536 bp in length, which totally contains 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a non-coding region (putative control region, CR). The phylogeny indicates that F. fuscipennis of subfamily Trypetinae was monophyletic and clearly separated from both Dacinae and Tephritinae with high bootstrap value supported.
RESUMO
The melon fly, Zeugodacus cucurbitae (Diptera:Tephritidae) is an important invasive pest and distributed throughout tropical, subtropical countries and areas. In this study, we report the complete mitochondrial genome of the fly with 15, 685 base pair in length, which includes 37 genes (the large and small ribosomal RNA subunits, 22 transfer RNA genes, 13 genes encoding mitochondrial proteins) and a non-coding A + T-rich control region. Molecular phylogeny indicated that there was a high bootstrap value supported among Z. cucurbitae and Z. tau belonging to the Z. tau complex.
RESUMO
Carpomya vesuviana (Diptera: Tephritidae), commonly known as the ber fruit fly, is the most destructive insect pest of 'ber' (jujube) throughout Asia and Western Europe. Complete sequence of the mitogenome of C. vesuviana has been determined in this study. The circular genome is 15,267 bp long and contains a standard gene complement, that is, the large and small ribosomal RNA subunits, 22 transfer RNA genes, 13 genes encoding mitochondrial proteins, and a non-coding A + T-rich control region. The phylogeny showed that C. vesuviana of subfamily Trypetinae was monophyletic and clearly separated from both Dacinae and Tephritinae with maximum support (p = 1).
RESUMO
Acrotaeniostola dissimilis (Diptera: Tephritidae) is an insect pest of bamboo shoot and distributed in the Yunnan and Sichuan Provinces, Southwest China. Complete mitogenome sequence has been determined in this study. The circular genome is 15384 bp long and contains a standard gene complement, that is, the large and small ribosomal RNA subunits, 22 transfer RNA genes, 13 genes encoding mitochondrial proteins, and a non-coding A + T-rich control region. The phylogeny showed that A. dissimilis in tribe Gastrozonini was a monophyletic branch and clearly separated from both tribe Dacini and tribe Ceratitidini with high bootstrap value supported.
RESUMO
Chinese black ant, Polyrhachis dives Smith, also known as P. vicina Roger has been used as a traditional edible insect and Chinese medicine in China and some southeast countries for thousands of years. In this study, Mitogenome of this species was assembled with high coverage using Illumina sequencing data and is 15,806 bp long in size. The base composition is 37.8% for A, 12.5% for C, 6.4% for G and 43.3% for T. The mitogenome contains 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. The phylogeny showed that it was closely related to Camponotus atrox (Hymenoptera: Formicidae) with high bootstrap value supported. The mitogenome of P. dives can provide essential DNA molecular data for further phylogenetic and evolutionary analysis.
RESUMO
Bactrocera correcta (Diptera: Tephritidae) is one of the most serious pest insects in south China and surrounding Southeast Asian countries. The family Tephritidae includes over 4257 species distributed worldwide, so the complete mitochondrial genome would be helpful for bio-identification, biogeography and phylogeny. The B. correcta genome consists of 15 936 bp. Annotation indicated that the structure and orientation of 13 protein-coding genes (PCGs), 22 tRNA and 2 rRNA sequences were typical of, and similar to, the ten closely related tephritid species. The nucleotide composition shows heavily biased toward As and Ts accounting 73.2% and exhibits a slightly positive AT skew, which is similar to other known tephritid species and other insects. The phylogenetic tree indicated the presence of three distinct families (Tephritidae, Muscidae, Drosophilidae) in Order Diptera.